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mom blocker  (Vector Laboratories)


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    Structured Review

    Vector Laboratories mom blocker
    Mom Blocker, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1531 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mom+blocker/Mouse+on+Mouse+(M%2EO%2EM%2E+)+Blocking+Reagent/pmc07062219-216-10-13
    Average 96 stars, based on 1531 article reviews
    mom blocker - by Bioz Stars, 2026-09
    96/100 stars

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    Incubation:

    Article Title: A Conjugate of a Tumor-Targeting Ligand and a T Cell Costimulatory Antibody To Treat Brain Tumors
    Article Snippet: T cell immunotherapy is a potential strategy for the treatment of brain tumors because it offers a high degree of specificity, the ability to extravasate into solid tumors, and the potential for eliciting a long-term protective immune response.. Various approaches have been developed to overcome T cell immune tolerance to cancer, including the use of cytokines and bispecific antibodies.. T cell stimulation with the proinflammatory cytokine IL-12 can elicit antitumor immunity.

    Labeling:

    Article Title: Aldosterone Regulates Pendrin and Epithelial Sodium Channel Activity through Intercalated Cell Mineralocorticoid Receptor–Dependent and –Independent Mechanisms over a Wide Range in Serum Potassium
    Article Snippet: .. For MR labeling, sections were blocked for 1 hour in MOM blocker (MKB-2213; Vector Laboratories) with an unconjugated Fab fragment goat anti-mouse IgG (115–007–003; Jackson ImmunoResearch, West Grove, PA), and for 15 minutes with Serum-Free Protein Block (X0909; Agilent Dako, Santa Clara, CA). .. Sections were then incubated at 4°C overnight with primary antibody diluted in Dako antibody diluent, washed in PBS, and incubated for 45 minutes in polymerlinked peroxidase-conjugated anti-mouse IgG diluted to 1:10 or 30 minutes in anti-rabbit IgG (Vector ImmPRESS), washed again with PBS, and exposed to diaminobenzidine (Vector DAB substrate kit) for 5 minutes.

    Article Title: Aldosterone Regulates Pendrin and Epithelial Sodium Channel Activity through Intercalated Cell Mineralocorticoid Receptor–Dependent and –Independent Mechanisms over a Wide Range in Serum Potassium
    Article Snippet: .. For MR labeling, sections were blocked for 1 hour in MOM blocker (MKB-2213; Vector Laboratories) with an unconjugated Fab fragment goat anti-mouse IgG (115–007–003; Jackson ImmunoResearch, West Grove, PA), and for 15 minutes with Serum-Free Protein Block (X0909; Agilent Dako, Santa Clara, CA). .. Sections were then incubated at 4°C overnight with primary antibody diluted in Dako antibody diluent, washed in PBS, and incubated for 45 minutes in polymer-linked peroxidase-conjugated anti-mouse IgG diluted to 1:10 or 30 minutes in anti-rabbit IgG (Vector ImmPRESS), washed again with PBS, and exposed to diaminobenzidine (Vector DAB substrate kit) for 5 minutes.

    Blocking Assay:

    Article Title: Aldosterone Regulates Pendrin and Epithelial Sodium Channel Activity through Intercalated Cell Mineralocorticoid Receptor–Dependent and –Independent Mechanisms over a Wide Range in Serum Potassium
    Article Snippet: .. For MR labeling, sections were blocked for 1 hour in MOM blocker (MKB-2213; Vector Laboratories) with an unconjugated Fab fragment goat anti-mouse IgG (115–007–003; Jackson ImmunoResearch, West Grove, PA), and for 15 minutes with Serum-Free Protein Block (X0909; Agilent Dako, Santa Clara, CA). .. Sections were then incubated at 4°C overnight with primary antibody diluted in Dako antibody diluent, washed in PBS, and incubated for 45 minutes in polymerlinked peroxidase-conjugated anti-mouse IgG diluted to 1:10 or 30 minutes in anti-rabbit IgG (Vector ImmPRESS), washed again with PBS, and exposed to diaminobenzidine (Vector DAB substrate kit) for 5 minutes.

    Article Title: Reduced SMN protein in motor neuronal progenitors functions cell autonomously to cause spinal muscular atrophy in model mice expressing the human centromeric (SMN2) gene
    Article Snippet: SMN expression in spinal motor neurons of transgenic mice was assessed by co-staining with antibodies against ChAT (see above) and SMN (1:500; BD Biosciences). .. The protocol was identical to the one described above except that an additional 1hr blocking step using MOM blocker (Vector Laboratories) was included to prevent the secondary antibody from binding to non-specific mouse antigens. ..

    Article Title: Aldosterone Regulates Pendrin and Epithelial Sodium Channel Activity through Intercalated Cell Mineralocorticoid Receptor–Dependent and –Independent Mechanisms over a Wide Range in Serum Potassium
    Article Snippet: .. For MR labeling, sections were blocked for 1 hour in MOM blocker (MKB-2213; Vector Laboratories) with an unconjugated Fab fragment goat anti-mouse IgG (115–007–003; Jackson ImmunoResearch, West Grove, PA), and for 15 minutes with Serum-Free Protein Block (X0909; Agilent Dako, Santa Clara, CA). .. Sections were then incubated at 4°C overnight with primary antibody diluted in Dako antibody diluent, washed in PBS, and incubated for 45 minutes in polymer-linked peroxidase-conjugated anti-mouse IgG diluted to 1:10 or 30 minutes in anti-rabbit IgG (Vector ImmPRESS), washed again with PBS, and exposed to diaminobenzidine (Vector DAB substrate kit) for 5 minutes.

    Binding Assay:

    Article Title: Reduced SMN protein in motor neuronal progenitors functions cell autonomously to cause spinal muscular atrophy in model mice expressing the human centromeric (SMN2) gene
    Article Snippet: SMN expression in spinal motor neurons of transgenic mice was assessed by co-staining with antibodies against ChAT (see above) and SMN (1:500; BD Biosciences). .. The protocol was identical to the one described above except that an additional 1hr blocking step using MOM blocker (Vector Laboratories) was included to prevent the secondary antibody from binding to non-specific mouse antigens. ..



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